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SonicMan is a high
throughput multi-probe sonication instrument developed by MatriCal,
configurable with 96, 384, and 1536 well formats. SonicMan uses disposable
pin lids to transfer sonic energy to each individual well and to prevent
well-to-well cross contamination.
Plates are placed on a retractable shuttle. The sonicator provides variable
power outputs up to 1150 W and configurable time intervals from 0.1 to 20
seconds.
- High throughput DNA
shearing - in 4 hours SonicMan can shear 96 samples to smears centered on
150 with nothing above ~250 bp
- Fragment size is
correlated with sonication settings (power/time) and is controllable
by the user. Fragments centered around a desired length can be
repeatedly generated in seconds
- Samples may be sonicated
in volumes ranging from 25ul to 1.5ml per well. Formats are scalable
to 96-well and 384-well plates
- Unlike enzyme based
digestion, the random fragments generated are suitable for the
sequencing of large genomes
- Sonication is an
uncomplicated, quick, process compared with techniques like
hydrodynamic shearing
- No specialized reagents
are needed minimizing solution variation and providing easy carryover
to downstream applications
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MORE
INFORMATION
SonicMan can operate standalone or integrated into an automated
workstation.
Other applications of SonicMan include; extraction of DNA, RNA, proteins,
enzymes etc by cell lysis, transfection and resuspension/dissolution of
compounds.
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