dna science and technology

Knowledge of DNA sequences of genes and other parts of the genome of organisms has become indispensable for basic research studying biological processes, as well as in applied fields such as diagnostic or forensic research. The advent of DNA sequencing has significantly accelerated biological research and discovery. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of the human genome, in the Human Genome Project.

The separation of dna for the host cells, sample preparation, quantitation, amplification and analysis is described at DOE Joint Genome Institute.

see also dna fragmentation

paa have been involved with DNA laboratories for over 15 years. paa were one of the original suppliers to the National DNA Database at the Forensic Science Service (FSS, UK).

The FSS were looking for piece of software that could control their small and large automated workcells, and OVERLORD Workstation (now Overlord3) was the chosen product. paa helped design and build the multiple automated workstations, both sample preparation and quantitation prior to amplification (left) and pre-analysis sample preparation prior to analysis by slab gel electrophoresis. Further information on forensic science applications

Overlord is still used in the FSS laboratories for user instrument interaction, providing a standard software platform for all interactions with equipment in the laboratories. Overlord also provides complete sample and plate tracking to a central LIMS for legal and logistic purposes.

paa have a complete range of tools for the scientist working on dna projects:

Liquid handling is a key to most aspects of DNA sample preparation and analysis tasks. Overlord3 supports a wide range of liquid handlers, however we have found the cost effective automate.it TPS range of liquid pipetting stations to suited for most liquid handling neds such as:

• siRNA screening
• RNAi assays
• PCR sample preparation
• normalization
• cherry picking
• plate replication
• serial dilution

Control software, world renown control software, Overlord3 offer easy-to-use interfaces for small and large automated workcells.

Integrated automation workcells, automate.it safecel automation workcells for protection of user and sample.

Integrated automated solution, completely integrated automation, solutions for any application with your choice of instruments into the workcell, see our extensive list

Sample tracking, use the automate.it storstar for sample tracking from sample reception through to the laboratory and Overlord3 with barcode technology to track the samples through the process.

Data handling, data handling flexibility unrivalled in laboratory automation control software for bespoke or commercial data handling packages such as Activity Base and Exegetix Biopower.

Small-insert random DNA fragments are necessary to create efficient libraries for genomic sequencing projects. The success and efficiency of sequencing a large genome is dependent on the randomness of the fragments generated by the shearing of target DNA. Physical shearing methods (i.e., sonication, nebulization, and hydrodynamic shearing are preferentially chosen over enzymatic digestion due to the randomness and size of the fragments produced resulting in a suitable overlapping collection of fragments for sub-cloning. The Matrical SonicMan™ offers these properties in a high-throughput sonication format allowing for a straightforward, user-friendly, customizable method to generate random DNA fragments. More Matrical SonicMan™ details, equipment & results