ForteBio Octet Red 384 automation

Automation of kinetic association and dissociation of biomolecules with an automate.it scara robot, barcode reader, Overlord3 laboratory automation control software and the ForteBio Octet RED 384 plate analysis instrument.

There are two key options, microplates can be stacked or placed in a rack.

• The stacked plates are usually for read once. Each plate is collected, the barcode read, and then passed to the instrument for
• The plates in the racks can be reread, n times at t time intervals. Different plates can be set up for different time intervals.

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Automation kinetic analysis of the association and dissociation of biomolecules can be performed on up to 384 samples in one 384-well plate. Automation requires the system to track each plate and the microplates that are transferred to and from the instrument at predetermined time intervals.

The biosensor, sample and reagent trays can be transferred to and from the Octet instrument by robot. Target ligands can be batch immobilized onto biosensors and returned to the biosensor tray for re-use in future experiments, thus extending the number of uses for each biosensor, preserving valuable ligand, and maximizing flexibility in experimental design.

Octet systems are currently used for life science research, including drug discovery and development, antibody characterization, proteomics, and quality control in biopharmaceutical manufacturing. With the Octet QK384 and Octet RED384 instruments, unmet needs in high-throughput quantitation and kinetic characterization are fulfilled.

• integrated instruments for analyzing biomolecular interactions
• full kinetic study support
• cost effective solution
• available as a complete system or an system upgrade

• increase throughput, reduce user interaction
• 24/7 operation of long studies
• improve the efficiency of your organization
• choose when you implement the automation stage of your project
• expert support

ForteBio’s Octet® family of instruments provides label-free, real-time measurements in the analysis of protein:protein, protein:peptide, and protein:small molecule interactions. With unparalleled ease of use, the Octet family of instruments moves your workflow beyond the limitations of existing label-free methods. You get comprehensive characterization across a broad range of applications that are critical for efficient development and production.

Kinetic Characterization The kinetic analysis of antibodies is critical to both the screening and selection of clones for development and thorough characterization throughout therapeutic development and production. With a dip and read label-free technique, ForteBio’s BLI-based systems are easy to learn. They also enable expanded kinetics analyses in a workflow that provides higher throughput without the limitations of flow-cell based systems Quantitative kinetics: characterization of ka, kd, and KD without flow-cell fluidics Kinetic screening: 96 measurements in less than two hours Increased throughput: eight-channel parallel analysis, 96 samples automated Assay crude samples: eliminate time for sample purification or preparation see ForteBio website Kinetic Characterization for more details Small Molecule and Small Peptides The Octet RED384 and Octet RED96 systems offer high sensitivity and rapid data acquisition capabilities that enable the analysis of low molecular weight molecules with fast interaction rates — in a flexible platform that is easy to learn and more affordable in comparison to existing systems with similar capabilities.   see ForteBio website Small Molecule and Peptides on the ForteBio website

Protein Quantitation in Bioprocessing Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with existing methods such as HPLC and ELISA. Early cell culture screening Protein purification Cell line selection for optimization of antibody production Biologicals manufacturing monitoring see ForteBio website Protein Quantitation in Bioprocessing for more details Qualitative Kinetics Assays Qualitative, real-time assays provide invaluable insight into biomolecular interactions that endpoint assays such as ELISA, cannot. With real-time binding sensorgrams and eight to sixteen-channel parallel analysis, the Octet family of instruments provides a qualitative tool for examining epitope binning, antibody isotyping, and screening of expression clones. This technology can deliver these assays: Epitope Isotyping Selection of Binding Pairs see ForteBio website Qualitative Kinetics Assays for more details

To install a system like this you would require:

• ForteBio Octet RED 384 instrument
• automate.it scara robot
• automate.it barcode reader
• Overlord3 laboratory automation control software

paa and Overlord3 support the full range of ForteBio instruments:

• ForteBio Octet QK384/RED384
• ForteBio Octet RED96
• ForteBio Octet QKe/QK

The paa automate.it harmony package includes:

• automate.it scara plate handler with a portrait gripper and 4 Stacks providing 90 SBS MTP capacity
  
• Overlord3™ scheduling software with instrument sockets to control the automate.it scara robot, LightCycler and any of our 405 supported instruments.
  
• Customer-specific data management configuration
  
• Installation of the system at the customer site.
  
• On-site acceptance testing and training.
  
• Full documentation including manuals.
  
• 1 year warranty with on-site support.

A customer, based in continental Europe, approached paa to discuss a collaborative project to integrate an existing ForteBio Octet RED 384 instrument into an automated workcell. The collaboration involved paa supplying the automate.it scara robot and Overlord3 control software and the customer writing the ForteBio Octet RED 384 instrument driver. After full customer training, the customer is able to develop kinetic assays to fully utilize the ForteBio Octet RED 384 instrument features.