Other Nanopipetting in laboratory automation

Reformatting compound libraries is a major requirement of the pharmaceutical laboratory. Nanolitre/Nanoliter transfers from mother-plate to daughter-plate eliminate the need for serial dilutions and improve accuracy and precision while saving time and money by eliminating consumables and washes.

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categories Genomic Solutions Innovadyne Labcyte Redd & Whyte Thermo TTP

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Automated Nanodispensing with the Labcyte Echo 550

Poster: AZ Process improvements in cell screening through introduction paa workcells and data analysis tools (SBS 2011)

 

The Labcyte® Echo™ 550 compound reformatter transfers volumes down to 2.5 nanolitres using a state-of-the-art “touchless” technology based on focused acoustic energy. Monitoring the acoustic energy reflected from the sample allows the Echo 550 to measure the water content of dimethyl sulfoxide (DMSO) and the fluid depth in each well.

• Replicate and reformat compound libraries
• Compress or expand storage plate
  • From 384- or 1536-well Echo qualified source plates
  • To 96-, 384-, 1536- or 3456-well SBS compliant destination plates
• User-developed protocols include:
  • Variable volume transfers from any source well to any destination well (Cherry-picking)
  • IC50 applications reducing sample consumption while improving results
• Audit compound libraries for well volume and DMSO hydration

Using the automate.it laboratory robot and Overlord3, the Echo 550 can be automated and plates can be added and tracked sequentially through the system. Full reports of Echo 550 generated sample data and transfer information can be moved to other databases with Overlord3.

When controlled by Overlord3, the automate.it arm can load the Labcyte Echo 550 from traditional lop loading stacks, random access stacks, as well as incubators.

The user can feed the system with a worklist generated from a LIMS or data management system for cherry picking.

The workcell can be further integrated with a wide range of bulk dispensing pipetting stations to complete the dilution of the compounds as part of the fully automated process. Full sample tracking is a standard part of these automated systems.